7 Questions 64 Answers 0 Followers
Questions related from Daniel Dehany Scott
Hi all, I'm currently looking at the dynamics of P-bodies in human cultured cell lines/tissues by immunofluorescence. As a comparison to my positive cell lines, I was interested to use (if...
03 March 2017 1,529 3 View
Hi everyone, This may be a slightly (or extremely) stupid question, but: how many 5'-3' RNA ligase enzymes are there in the human genome? So far, the answer seems, surprisingly, to be one:...
05 May 2016 4,687 2 View
Hi all, I am currently investigating a protein that shows both nucleolar and nucleoplasmic staining, and one of the analyses I would like to conduct is to test whether the nucleoplasmic fraction...
01 January 2016 4,909 3 View
We are hoping to monitor phosphorylation of E2F1 by ATM/ATR at Ser31 by western blotting. However, while this modification has has been widely reported in the literature, I have not been able to...
11 November 2015 4,956 0 View
My lab is currently conducting assays on the sensitivity to ROS of a couple of cell lines, and we are looking at the different agents used to induce ROS. I've heard that tBHP (tert-Butyl...
05 May 2014 7,951 4 View
My lab is currently investigating the different cell cycle arrest phenotypes of a knocked-out gene of interest, and we were keen to see the effect of knocking out p27 in our genetic background...
05 May 2014 5,543 0 View
I have developed a database of members of our organisation; these members are drawn from about 150 countries around the world and are mostly from academia or para-academic professions (e.g....
09 September 2013 6,603 10 View
