We have recently generated CAR-Jurkat and CAR-pbmc cells, however, although the initial CAR% is around 80%, after 2 days of culture in vitro, the CAR expression dramatically falls to 20% or 0%,does anyone have any suggestions OR similar experience?
which promoter do you use? Some promoters will be silenced in cells.
How do you check if CAR is actually in the cells?
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