How to calculate stress at the cross section of spherical particles within a matrix?

Hi all, I am currently working on some background calculations before I start a new project related to metal matrix particulate composites. I am trying to find a way to calculate the stress at...

19 February 2021 8,503 3 View

Posthoc test after two-way ANOVA (two factors with two levels)?

Hi everyone, I was wondering if runnig a posthoc test regardeless of a significant interaction might make sense. I have prepared a dataset with obviously invented data but which can help to the...

02 February 2021 8,061 1 View

How can I use amylase enzyme to digest molluscan tissue before DNA extration?

Hi, I have a problem with a hight contet of mucopolysaccharides in some molluscan samples. I tried different DNA extration procolols but I can't already remove all the molecules that inhibit PCR...

01 January 2021 8,900 3 View

Will the spread of the new SARS-Cov-2 strain affect the efficacy of mRNA-based vaccines?

Recent studies have highlighted the isolation of a new strain of SARS-Cov-2. Could the new discovery compromise the effectiveness of the vaccination campaign ???

21 December 2020 7,516 4 View

Metasurface S parameter measure setup. How to do that?

Hi. I want to simulate the S mesurament setup of a metasurface. I took an horn antenna and simulated it's gain at my frequency. The I placed one on a side and the second on the opposite side....

16 October 2020 2,714 3 View

Is temperature measurement really useful as a screening measure for COVID-19?

The measurement of the temperature at the entrance to some structures or hospitals is a universally adopted measure in prevention strategies. There are two questions: considering the high number...

31 August 2020 5,843 12 View

Which could be a good DL technique for tracking an object and check for his integrity?

Hello everyone, I'm working on a computer vision project in which I need to detect in real time a moving object and check whether is still integer or not. Images will be supplied from a 4K...

05 August 2020 2,460 2 View

After the shocking case of George Floyd, should the physical restraint techniques used in the police and psychiatric settings be recoded?

George Floyd's case shocked the world. Physical restraint is a method used by the police but also in the management of psychiatric patients who pose a danger to themselves or others. In your...

10 June 2020 1,331 9 View

Can the political orientation of a medical / scientific journal editor influence scientific choices?If so, it'is morally and ethically acceptable?

Donald Trump's statements and the political opinion that publishers, especially American publishers, had of him profoundly indicated the editorial lines of the main scientific newspapers. Most...

10 June 2020 8,913 15 View

How to model in Arena Rockwell that all entities must clear their tails at the end of the simulation?

I am modeling the flow of visitors during a working day in a museum. The main problem is that I don't know how to model in Arena Rockwell the fact that all entities must clear the queue at the end...

20 February 2020 9,457 1 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View