I am interested in protocols and tips to perform organotypic cultures, especially from large animal models (e.g. Pig). Thanks
My collegue and I are having hard time to understand what is happening. Basically we have a tapping device that work with Arduino and a pneumatic pump. The pump allows the tapping of two...
04 August 2024 4,662 1 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
Hi! I'm simulating a specific material on Quantum Espresso, and I found something strange. Initially I've performed simulations on FCC InAs crystal and I used the Hubbard correction to reproduce...
02 July 2024 9,323 1 View
Let's suppose we have a series of time-based data describing a dynamic (e.g., air temperature over the year, measured once a day). Is it possible to apply a statistical technique that allows me to...
05 April 2024 1,591 2 View
Hello, When you want to investigate exacerbations (outcome of interest) in asthma, you can measure: 1) the incidence rate in the last year (i.e. mean numbers of exacerbations in the last year in...
14 December 2023 9,828 1 View
Hi everyone, Does anyone have a recipe for zebrafish aCSF that might match the molar concentrations reported by companies like Harvard Apparatus or Tocris (in mM Na 150; K 3.0; Ca 1.4; Mg 0.8; P...
03 December 2023 3,919 0 View
Hallo everyone, I need to ask for advice: I am currently doing a research on Carpal Tunnel Syndrome and Ultrasound measurements of the median nerve cross sectional area, in particular I am...
26 September 2023 7,638 0 View
Dear all, Thanking you in advance for your attention, I would like to ask you two questions regarding an electrophoretic run on 2% agarose gel: 1) I did an electrophoretic run of the Real-time...
25 June 2023 7,501 4 View
Hello everyone, I am working on my Phd on free surface water streams with conda effect. Basically I am acquiring images from above of a rectangular basin in which enters a jet which is then...
22 June 2023 5,089 0 View
Dear RG staff I'm Simone Seghetti. I'm writing to you for an issue about an article which I had incorrectly uploade (following...
18 April 2023 9,797 3 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View