Does anyone has experience with indirect organogenesis of Vitis vinifera. I mean shoot organogenesis from calli (not somatic embryogenesis).
I suggest you contact Prof Ebadi ([email protected]) who has experience.
some times plants produces auxin in media when they stay long time and so produce roots.
I'm starting with Croatian native cultivars screening on suitable conditions for organogenesis from cali developed from green internodal segments. Which hormone combinations and concentration should be included in such a experiment (BA, IBA, IAA, TDZ..). Light and temperature conditions? I am planning to use MS and ½ MS media.
Dobar dan, Darko!
I've had no personal experience with V. vinifera indirect organogenenis, but I would try the methof of Mezzetti et al (BMC Biotechnology, 2002 http://www.biomedcentral.com/content/pdf/1472-6750-2-18.pdf ) who developed an organogenesis protocole for grape transformation. Seems to work well enough, although it takes some times to obtain the regenerative tissue from apical meristems. Shoot formation from wounded in vitro leaves seems interesting too although it didn't seem to work with vinifera cultivars (http://download.springer.com/static/pdf/222/art%253A10.1007%252FBF00272104.pdf?auth66=1414749722_13b8a4a2ca69e14eaa2b0441f1e2a700&ext=.pdf), but I would give it another try modifying the GR...
I'm not aware of papers describing shoot regeneration in grapevine from internode callus, remember that not all calli have organogenic abilities..
Good luck..
I concur with the previous responder, however response to plant growth regulators is species even cultivar dependent. What has been reported by others gives you the starting point towards optimization of the protocol for that specific V.vinifera.
Contact Dr. S. Dhekney of University of Wyoming, USA. You can get information on him on google.
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