Hello..please i did run MD simulations for 50 ns and the results i had for my radius of gyration doesn't look good. can someone please tell me where the problem is coming from and how to solve it..Thanks
Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...
25 May 2024 5,979 3 View
I perform al alkylation reaction on an imdazole compound where the alkyl reagent (1-Iodo-Octane) was in excess. After the reaction was completed, I then performed an extraction, followed by...
24 May 2024 8,042 2 View
Hello, I'm exploring whether incorporating edge weights in a network affects the extent to which the network is partitioned into clusters of closely connected nodes. Specifically, I'm...
13 May 2024 5,078 0 View
We have evaluated two stakeholders networks of two same type of events in regard three different criteria (communication, collaboration and knowledge exchange). Using UCINET software we have...
27 March 2024 2,348 0 View
I was told by a lab colleague that 'after purifying a protein with a his-tag using imidazole, it is crucial to quickly remove the imidazole. Keeping the protein in more than 200mM of imidazole is...
04 March 2024 4,892 1 View
Hi all, Do you do DO calibration while the production is ongoing? Typically I don't do it. However, my recent data showed that offline pO2 is extremely high (approximately 300mmHg) but the...
22 December 2023 3,270 0 View
I want help on design the draw profile daily on domestic water (throughout the day) using TRNSYS. Please your help !!!
29 November 2023 1,163 0 View
I'm working on isolating recombinant protein in E.coli (Ni-NTA) I'am trying to purify recombinant protein that I overexpressed E.coli BL21(DE3)pLysS using the pCold1 vector. The proteins have...
03 November 2023 3,064 3 View
I'm working on preparation of protein lysates from E.coli I read in a few paper that magnesium acetate should be added before sonication what is role of the Mg ace in cell lysis?
03 November 2023 4,891 2 View
I do not doubt climate change and the disasters that come and will come from it (Some thoughts on climate change, resource constraints, and adaptation ). However, one thing puzzles me: why would...
28 October 2023 4,607 8 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,952 0 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,032 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,743 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 486 0 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,571 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...
24 July 2024 8,172 1 View
Hello everyone, I am conducting
22 July 2024 8,085 11 View
Hi, We have collected several images for two independent runs of an experiment using two different confocal microscopes and settings. Thus, the intensity values collected through ImageJ were...
22 July 2024 8,720 0 View
Actually, I'm having trouble implementing the plot of the flutter region for the whirl prop system with 2 DOF (Influences of structural damping and propeller—pivot point distance on whirl flutter...
21 July 2024 5,043 2 View