AcrD pump in BL21 cells
I am trying to characterize the secondary structure of my aptamer . I don't see neat and clear digested bands in my samples. I am attaching all the gels I have run until now, this is my first...
05 June 2014 3,932 1 View
I am trying to induce angiogenesis in Adult Bovine aortic endothelial cells using Matrigel at a concentration of 37.5ug/ml. I wanted to confirm if the cells are actually showing lumen formation. I...
11 December 2013 5,556 8 View
I am trying to separate RNA binding sequences to virus particles from non-binders through sucrose centrifugation. I am layering the RNA virus particle mix onto 20% sucrose and taking the pellet...
01 February 2013 8,250 7 View
I am selecting RNA aptamers against a viral protein. To increase its nuclease resistance we use 2' fluoro modified RNA. I am wondering if the 2' fluoro modification has any affect in the...
31 December 2012 4,669 0 View
I am working with bovine aortic endothelial cells and after thawing I split them at 1:3 ratio. The cells didn't grow, so I came across an article saying that BAEC's divide in the presence of fgf....
09 October 2012 1,833 13 View
I have hit a roadblock in my qPCR reactions. I am trying to quantify the mRNA levels of PAI-1 gene in BAECs . I see jagged amplification peaks in my qPCR. I thought might be my cDNA is not good...
05 June 2012 8,336 9 View
I am working with Bovine Aortic endothelial cells and in those cells we want to check the effect of growth factors on a particular gene at the mRNA levels. There are human primary cells available...
05 June 2012 6,014 95 View
My end goal of the experiment is to do a qPCR of the RNA samples. I isolated RNA but didnt get very good yields and also the 230 peak was very high. Still, I went ahead with reverse transcription...
04 May 2012 2,553 6 View
Hello Everyone, I am working with fetal bovine aortic endothelial cells and I treat them with specific growth factors eventually. Usually they are very elongated and have protusions when dividing...
04 May 2012 9,836 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
