AcrD pump in BL21 cells
I am trying to characterize the secondary structure of my aptamer . I don't see neat and clear digested bands in my samples. I am attaching all the gels I have run until now, this is my first...
05 June 2014 3,884 1 View
I am trying to induce angiogenesis in Adult Bovine aortic endothelial cells using Matrigel at a concentration of 37.5ug/ml. I wanted to confirm if the cells are actually showing lumen formation. I...
11 December 2013 5,503 8 View
I am trying to separate RNA binding sequences to virus particles from non-binders through sucrose centrifugation. I am layering the RNA virus particle mix onto 20% sucrose and taking the pellet...
01 February 2013 8,202 7 View
I am selecting RNA aptamers against a viral protein. To increase its nuclease resistance we use 2' fluoro modified RNA. I am wondering if the 2' fluoro modification has any affect in the...
31 December 2012 4,627 0 View
I am working with bovine aortic endothelial cells and after thawing I split them at 1:3 ratio. The cells didn't grow, so I came across an article saying that BAEC's divide in the presence of fgf....
09 October 2012 1,787 13 View
I have hit a roadblock in my qPCR reactions. I am trying to quantify the mRNA levels of PAI-1 gene in BAECs . I see jagged amplification peaks in my qPCR. I thought might be my cDNA is not good...
05 June 2012 8,150 9 View
I am working with Bovine Aortic endothelial cells and in those cells we want to check the effect of growth factors on a particular gene at the mRNA levels. There are human primary cells available...
05 June 2012 5,901 95 View
My end goal of the experiment is to do a qPCR of the RNA samples. I isolated RNA but didnt get very good yields and also the 230 peak was very high. Still, I went ahead with reverse transcription...
04 May 2012 2,507 6 View
Hello Everyone, I am working with fetal bovine aortic endothelial cells and I treat them with specific growth factors eventually. Usually they are very elongated and have protusions when dividing...
04 May 2012 9,788 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
