Can you help me with my problems with footprinting gel?

06 June 2014 1 4K Report

I am trying to characterize the secondary structure of my aptamer . I don't see neat and clear digested bands in my samples. I am attaching all the gels I have run until now, this is my first experience with these gels so any suggestion would be really helpful. Size of my RNA is 100 bases and I am running the gel at 1.5kV and 60mA.

Amanda O'Donnell

Perhaps you could post your protocol. Its difficult to tell what went wrong just from gels. I suspect you have several issues here.

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