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Questions related from Shambhavi Shubham
I am trying to characterize the secondary structure of my aptamer . I don't see neat and clear digested bands in my samples. I am attaching all the gels I have run until now, this is my first...
06 June 2014 3,910 1 View
I am trying to induce angiogenesis in Adult Bovine aortic endothelial cells using Matrigel at a concentration of 37.5ug/ml. I wanted to confirm if the cells are actually showing lumen formation. I...
12 December 2013 5,530 8 View
AcrD pump in BL21 cells
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I am trying to separate RNA binding sequences to virus particles from non-binders through sucrose centrifugation. I am layering the RNA virus particle mix onto 20% sucrose and taking the pellet...
02 February 2013 8,228 7 View
I am selecting RNA aptamers against a viral protein. To increase its nuclease resistance we use 2' fluoro modified RNA. I am wondering if the 2' fluoro modification has any affect in the...
01 January 2013 4,650 0 View
I am working with bovine aortic endothelial cells and after thawing I split them at 1:3 ratio. The cells didn't grow, so I came across an article saying that BAEC's divide in the presence of fgf....
10 October 2012 1,813 13 View
I have hit a roadblock in my qPCR reactions. I am trying to quantify the mRNA levels of PAI-1 gene in BAECs . I see jagged amplification peaks in my qPCR. I thought might be my cDNA is not good...
06 June 2012 8,211 9 View
I am working with Bovine Aortic endothelial cells and in those cells we want to check the effect of growth factors on a particular gene at the mRNA levels. There are human primary cells available...
06 June 2012 5,977 95 View
My end goal of the experiment is to do a qPCR of the RNA samples. I isolated RNA but didnt get very good yields and also the 230 peak was very high. Still, I went ahead with reverse transcription...
05 May 2012 2,532 6 View
Hello Everyone, I am working with fetal bovine aortic endothelial cells and I treat them with specific growth factors eventually. Usually they are very elongated and have protusions when dividing...
05 May 2012 9,815 3 View
