I just want to know if it works fine and produces good yield.
Is it feasible to extract Plasmodium DNA from infected whole blood samples?
I am working on beta glucuronidase and found a completely new class of potent inhibitors as revealed from their IC 50 values. however, I am having difficulty in establishing kinetics for these...
05 June 2017 2,197 9 View
I have a 15N labelled protein sample with the final concentration of 1mM. The spectra will be recorded using 800MHz NMR. I am unsure about the final concentration of DSS to be added as internal...
03 April 2017 2,023 3 View
I have cloned, expressed, and purified an hypothetical protein (13 KDa) from S. aureus using pET 25b expression vector. Protein contains 1 cysteine residue which may accounts for dimerization. To...
04 May 2014 9,310 5 View
I am working on Methicillin resistant Staphylococcus aureus MRSA proteins and would like to know about designing functional studies for hypothetical proteins. Kindly suggest me the softwares and...
31 December 2013 5,002 6 View
I have cloned my gene of interest in pSpeedET using NgoMIV and Xba1 restriction enzyme. Gene was isolated via PCR using gene specific primers with restriction site over hangs at 5' ends. After...
08 September 2013 9,095 5 View
I am facing problems of non specific amplification in my colony PCR experiments. My specific product is present but its band is not as intense compared to the non specific mesh that appears below...
05 June 2013 8,087 14 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View