I just want to know if it works fine and produces good yield.
Is it feasible to extract Plasmodium DNA from infected whole blood samples?
I am working on beta glucuronidase and found a completely new class of potent inhibitors as revealed from their IC 50 values. however, I am having difficulty in establishing kinetics for these...
05 June 2017 2,304 9 View
I have a 15N labelled protein sample with the final concentration of 1mM. The spectra will be recorded using 800MHz NMR. I am unsure about the final concentration of DSS to be added as internal...
03 April 2017 2,143 3 View
I have cloned, expressed, and purified an hypothetical protein (13 KDa) from S. aureus using pET 25b expression vector. Protein contains 1 cysteine residue which may accounts for dimerization. To...
04 May 2014 9,479 5 View
I am working on Methicillin resistant Staphylococcus aureus MRSA proteins and would like to know about designing functional studies for hypothetical proteins. Kindly suggest me the softwares and...
31 December 2013 5,099 6 View
I have cloned my gene of interest in pSpeedET using NgoMIV and Xba1 restriction enzyme. Gene was isolated via PCR using gene specific primers with restriction site over hangs at 5' ends. After...
08 September 2013 9,206 5 View
I am facing problems of non specific amplification in my colony PCR experiments. My specific product is present but its band is not as intense compared to the non specific mesh that appears below...
05 June 2013 8,203 14 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View