I have a 15N labelled protein sample with the final concentration of 1mM. The spectra will be recorded using 800MHz NMR. I am unsure about the final concentration of DSS to be added as internal standard.
Check this links out
http://www.mdpi.com/1420-3049/22/4/559/htm
https://www2.chemistry.msu.edu/facilities/nmr/900mhz/MCSB_NMR_sample.html
As you can see here: http://bayesil.ca/spectra_collection a 1 mM final concentration is good enough!
Thanks,
Biswa
Thank you guys.
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