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Questions related from Shezaib Siddiqui
I am working on beta glucuronidase and found a completely new class of potent inhibitors as revealed from their IC 50 values. however, I am having difficulty in establishing kinetics for these...
06 June 2017 2,273 9 View
I have a 15N labelled protein sample with the final concentration of 1mM. The spectra will be recorded using 800MHz NMR. I am unsure about the final concentration of DSS to be added as internal...
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I have cloned, expressed, and purified an hypothetical protein (13 KDa) from S. aureus using pET 25b expression vector. Protein contains 1 cysteine residue which may accounts for dimerization. To...
05 May 2014 9,432 5 View
I am working on Methicillin resistant Staphylococcus aureus MRSA proteins and would like to know about designing functional studies for hypothetical proteins. Kindly suggest me the softwares and...
01 January 2014 5,069 6 View
I just want to know if it works fine and produces good yield. Is it feasible to extract Plasmodium DNA from infected whole blood samples?
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I have cloned my gene of interest in pSpeedET using NgoMIV and Xba1 restriction enzyme. Gene was isolated via PCR using gene specific primers with restriction site over hangs at 5' ends. After...
09 September 2013 9,168 5 View
I am facing problems of non specific amplification in my colony PCR experiments. My specific product is present but its band is not as intense compared to the non specific mesh that appears below...
06 June 2013 8,165 14 View