Shezaib Siddiqui

7 Questions 21 Answers 0 Followers

Questions related from Shezaib Siddiqui

What kind of Enzyme Inhibition is it?

I am working on beta glucuronidase and found a completely new class of potent inhibitors as revealed from their IC 50 values. however, I am having difficulty in establishing kinetics for these...

06 June 2017 2,261 9 View

How much final concentration of DSS should be used as internal standard for protein NMR?

I have a 15N labelled protein sample with the final concentration of 1mM. The spectra will be recorded using 800MHz NMR. I am unsure about the final concentration of DSS to be added as internal...

04 April 2017 2,095 3 View

How can I prevent protein dimerization?

I have cloned, expressed, and purified an hypothetical protein (13 KDa) from S. aureus using pET 25b expression vector. Protein contains 1 cysteine residue which may accounts for dimerization. To...

05 May 2014 9,414 5 View

Ideas for experimental design for functional studies?

I am working on Methicillin resistant Staphylococcus aureus MRSA proteins and would like to know about designing functional studies for hypothetical proteins. Kindly suggest me the softwares and...

01 January 2014 5,058 6 View

Does anyone have experience with Bio Basic blood DNA extraction kit (BS684)?

I just want to know if it works fine and produces good yield. Is it feasible to extract Plasmodium DNA from infected whole blood samples?

01 January 2014 3,441 0 View

Could anyone suggest a protocol for an isolation of gene via PCR?

I have cloned my gene of interest in pSpeedET using NgoMIV and Xba1 restriction enzyme. Gene was isolated via PCR using gene specific primers with restriction site over hangs at 5' ends. After...

09 September 2013 9,154 5 View

Problem in colony PCR

I am facing problems of non specific amplification in my colony PCR experiments. My specific product is present but its band is not as intense compared to the non specific mesh that appears below...

06 June 2013 8,152 14 View