I am hoping to perform biolistic transfection of organotypic brain slice cultures. In my previous lab we used the Biorad Helios Gene Gun System - which effectively is just a pressurized gun that fires gold bullets through nylon tubing. However the system costs $30 000 which is exorbitant for the technology! Has anyone used alternatives such as this Chinese competitor: http://www.scientzbio.com/gene-transduction-device/portable-gene-gun-sj-500.html
Any thoughts would be welcome!
Hi,
I have not heard of that company personally but that doesn't mean anything.
I have however seen multiple labs succeed in building their own gene gun from scratch.
Attached is a reference that may help you get started if you choose to go that way.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441267/
And here is an easier to follow web article entirely on the topic of a homemade gene gun.
https://www.oreilly.com/ideas/how-to-build-and-use-a-gene-gun
Hi,
I have not heard of that company personally but that doesn't mean anything.
I have however seen multiple labs succeed in building their own gene gun from scratch.
Attached is a reference that may help you get started if you choose to go that way.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441267/
And here is an easier to follow web article entirely on the topic of a homemade gene gun.
https://www.oreilly.com/ideas/how-to-build-and-use-a-gene-gun
Hi Joe,
here's some more DIY links you may find useful:
Article Development of a low-pressure microtargeting biolistic devic...
and
http://2016.igem.org/Team:Cambridge-JIC/Biolistics
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