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Questions related from Joseph Valentino Raimondo
We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...
18 November 2020 2,980 1 View
We are making whole-cell patch clamp recordings from mouse (and human) fast-spiking interneurons using Axopatch 200B amplifiers. We see a sharp overshoot after action potentials (see red trace in...
31 January 2019 8,310 6 View
I am hoping to perform biolistic transfection of organotypic brain slice cultures. In my previous lab we used the Biorad Helios Gene Gun System - which effectively is just a pressurized gun that...
06 June 2018 1,971 3 View
We have homogenized Taenia crassiceps larvae and are puffing the homogenate onto pyramidal neurons during whole-cell patch-clamp recordings in organotypic hippocampal slice cultures. We need to...
23 May 2017 5,228 6 View
Dear all, I'm looking to purchase a new patch clamp amplifier. Axon instruments appears to be the gold standard (they're the only amplifiers I've ever used) but the Multiclamp 700B is about $17...
19 January 2016 8,393 20 View
I'm looking for advice on purchasing a vibratome for patch-clamp electrophysiology. We'd be making cortical and hippocampal brain slices. Currently we use a Vibratome Series 1000 which works...
02 October 2014 6,335 29 View
The Sutter P-97 is the workhorse of many electrophys labs. I'm looking to purchase a new horizontal puller. Does anyone have any experience with the new P-1000 - is it as reliable as the old P-97?...
26 August 2013 1,225 2 View
Is it possible to decontaminate a vibratome (slicer) that has previously been used on fixed tissue to be used to make fresh brain slices? Has anyone tried this? I've been told that even trace...
21 May 2013 6,986 16 View