Joseph Valentino Raimondo

7 Questions 27 Answers 0 Followers

Questions related from Joseph Valentino Raimondo

What is the best dye to use to visualise electroporation of neurons with DNA for later expression of a genetically-encoded fluorescent reporter?

We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...

18 November 2020 2,902 1 View

Should one use pipette capacitance correction when using an Axopatch 200B to make recordings from interneurons?

We are making whole-cell patch clamp recordings from mouse (and human) fast-spiking interneurons using Axopatch 200B amplifiers. We see a sharp overshoot after action potentials (see red trace in...

31 January 2019 8,270 6 View

Does anyone have experience with an alternative to the Biorad Helios Gene Gun system for biolistic transfection?

I am hoping to perform biolistic transfection of organotypic brain slice cultures. In my previous lab we used the Biorad Helios Gene Gun System - which effectively is just a pressurized gun that...

06 June 2018 1,910 3 View

We're puffing a homogenate of unknown composition onto a neuron during a whole-cell recording, what might cause the membrane depolarization we see?

We have homogenized Taenia crassiceps larvae and are puffing the homogenate onto pyramidal neurons during whole-cell patch-clamp recordings in organotypic hippocampal slice cultures. We need to...

23 May 2017 5,180 6 View

Patch-clamp amplifiers: Multiclamp 700B vs EPC 10 vs AM systems 2400?

Dear all, I'm looking to purchase a new patch clamp amplifier. Axon instruments appears to be the gold standard (they're the only amplifiers I've ever used) but the Multiclamp 700B is about $17...

19 January 2016 8,350 20 View

Can anyone provide advice on purchasing a vibratome for patch-clamp electrophysiology?

I'm looking for advice on purchasing a vibratome for patch-clamp electrophysiology. We'd be making cortical and hippocampal brain slices. Currently we use a Vibratome Series 1000 which works...

02 October 2014 6,284 29 View

Is the new Sutter P-1000 horizontal micropipette puller as reliable as the 'old' Sutter P-97 puller?

The Sutter P-97 is the workhorse of many electrophys labs. I'm looking to purchase a new horizontal puller. Does anyone have any experience with the new P-1000 - is it as reliable as the old P-97?...

26 August 2013 1,185 2 View