Joseph Valentino Raimondo

8 Questions 29 Answers 0 Followers

Questions related from Joseph Valentino Raimondo

What is the best dye to use to visualise electroporation of neurons with DNA for later expression of a genetically-encoded fluorescent reporter?

We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...

18 November 2020 2,987 1 View

Should one use pipette capacitance correction when using an Axopatch 200B to make recordings from interneurons?

We are making whole-cell patch clamp recordings from mouse (and human) fast-spiking interneurons using Axopatch 200B amplifiers. We see a sharp overshoot after action potentials (see red trace in...

31 January 2019 8,316 6 View

Does anyone have experience with an alternative to the Biorad Helios Gene Gun system for biolistic transfection?

I am hoping to perform biolistic transfection of organotypic brain slice cultures. In my previous lab we used the Biorad Helios Gene Gun System - which effectively is just a pressurized gun that...

06 June 2018 1,979 3 View

We're puffing a homogenate of unknown composition onto a neuron during a whole-cell recording, what might cause the membrane depolarization we see?

We have homogenized Taenia crassiceps larvae and are puffing the homogenate onto pyramidal neurons during whole-cell patch-clamp recordings in organotypic hippocampal slice cultures. We need to...

23 May 2017 5,237 6 View

Patch-clamp amplifiers: Multiclamp 700B vs EPC 10 vs AM systems 2400?

Dear all, I'm looking to purchase a new patch clamp amplifier. Axon instruments appears to be the gold standard (they're the only amplifiers I've ever used) but the Multiclamp 700B is about $17...

19 January 2016 8,400 20 View

Can anyone provide advice on purchasing a vibratome for patch-clamp electrophysiology?

I'm looking for advice on purchasing a vibratome for patch-clamp electrophysiology. We'd be making cortical and hippocampal brain slices. Currently we use a Vibratome Series 1000 which works...

02 October 2014 6,341 29 View

Is the new Sutter P-1000 horizontal micropipette puller as reliable as the 'old' Sutter P-97 puller?

The Sutter P-97 is the workhorse of many electrophys labs. I'm looking to purchase a new horizontal puller. Does anyone have any experience with the new P-1000 - is it as reliable as the old P-97?...

26 August 2013 1,231 2 View

Can a vibratome that has previously been used on fixed tissue be used to make live brain slices?

Is it possible to decontaminate a vibratome (slicer) that has previously been used on fixed tissue to be used to make fresh brain slices? Has anyone tried this? I've been told that even trace...

21 May 2013 6,991 16 View