Can anyone suggest how to design a transistor model in ansys circuit?

Can anyone suggest how to design a transistor model in ansys circuit. snp file with respect to different bias is available but from the datasheet I want to design transistor and observe it's...

05 March 2020 2,582 3 View

Which one is cheap diagnosis in FNAC and Ziehl-Neelsen Staing in the diagnosis of Tuberculous Lymphadenitis ?

FNAC is an assay which has high sensitivities when optimally selected is efficient and one of the most accurate frontline method for the diagnosis of Tuberculous Lymphadenitis. It is effectively...

23 January 2018 3,853 20 View

Can anyone provide me a suitable protocol for isolation of plant antimicrobial peptide?

Plants contains different types of peptides. Some of them are antimicrobial in nature which helps them fight against different microbes. If I want to isolate these peptides (Extracellular or...

08 November 2017 9,459 3 View

What should I do to pull a polar compound (Schiff base) up from the baseline on TLC?

I am trying to synthesize a schiff base by reacting aldehyde with amine, using DMSO as solvent (as it is only soluble in this solvent). Before doing TLC, the sample was diluted with ethyl acetate...

13 September 2017 8,372 3 View

How to avoid noise during image processing in Western Blot through chemiluminescence method?

We use ECL substrate and HRP enzyme.

08 August 2017 1,802 4 View

How long can GFP plasmid DNA withstand sonication without hampering its expression?

11 April 2017 2,179 3 View

Which simulation software is more reliable for antenna design as well as mesh analysis among Ansoft HFSS, CST Microwave Studio and ADS Agilent?

Antenna design in one software varies with result in another software. Also which one is better for mesh analysis.

13 October 2016 9,190 12 View

Can we use luciferase assay cell lysate for western blot? if anybody has used, please share protocol?

I have performed luciferase assay and I want to do western blot using the lysates.

28 January 2016 1,115 2 View

Looking for Electromagnetics Books by Hollis C. Chen and Branislav Notoras

Hello, Can anybody please suggest where I can find the pdf of this book? TIA. Theory of Electromagnetic Waves: A Coordinate-free Approach Author: Hollis C. Chen MATLAB-Based...

01 January 1970 2,764 8 View

What are the dis-advantages of porous metallic bi-polar plates for PEM fuel cell application ?

Please give some points regarding advantages and disadvantages of having porous bi-polar plates in PEM fuel cell.

01 January 1970 7,090 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View