13 August 2021 1 10K Report

I want to culture renal proximal tubule epithelial (RPTC) cells on a transparent membrane for microfluidic systems.

More Suruchi Poddar's questions See All
How much dynamic multiplier i should take for seismic zone IV to analyse diaphragm wall?

Hi, i am doing PhD and my research work is on dynamic analysis of diaphragm wall using plaxis 3D. For the analysis i want to know about dynamic multiplier. How much dynamic multiplier i should...

02 August 2022 7,568 0 View

Hi, I am performing seismic/ dynamic analysis on diaphragm wall on plaxis 3d and getting error code 110 in phase 3. how to resolve this error??

i have dynamic analysis on diaphragm wall in four phases. i have to eliminate error code 110 in phase 3, plz help me out

09 May 2022 5,775 0 View

Hi, I am getting a message in frequency calculation output file by using bp86/def2tzvp level of theory in gaussian 16.Can anyone give me the solution?

The message is as follows (the last portion of gaussian output file where the job has been stopped): Problem detected with inexpensive integrals. Switching to full accuracy and repeating last...

05 March 2022 2,533 3 View

In solute dispersion through different flow geometry, what is the background of using the absorption rate or boundary reaction is of first-order?

Numerous previous studies have shown that the absorption rate $\beta$ is of first-order, but what is the reason? Smith, R., 1983, Effect of boundary absorption upon longitudinal dispersion in...

19 June 2020 5,433 0 View

Can anyone suggest how to design a transistor model in ansys circuit?

Can anyone suggest how to design a transistor model in ansys circuit. snp file with respect to different bias is available but from the datasheet I want to design transistor and observe it's...

05 March 2020 2,775 3 View

How to set Lumped Port on substrate surface in HFSS?

I want to use Ansys co simulation feature. I have assigned port 1 and port 2 as lumped port with respect to ground. But I need to create a lumped port inside the ring gap. How can I do that.

06 February 2020 4,322 2 View

Immunodiagnosis of tuberculosis as a convenient and cost effective test to supplement clinical information for definite diagnosis ?

Tuberculosis is still a major health problem in most developing countries and its incidence is rising in many developed countries. This resurgence has been attributed to the HIV epidemic and TB...

13 February 2019 3,726 1 View

UGC’s decision to cut over 4,000 journals from approved list is arbitrary, lacks transparency ?

The academic community in India has been left shocked by the University Grants Commission’s decision last week to drop 4,305 journals from its list of over 38,000 officially approved publications....

13 May 2018 3,867 7 View

M. pneumoniae does produce typically fried-egg appearance colonies.Why?

1. Razin S. Mycoplasmas are spherical to filamentous cells with no cell walls. There is an attachment organelle at the tip of filamentous M pneumoniae, M genitalium, and several other pathogenic...

12 May 2018 2,915 1 View

Dual infection with HBV and HCV is not uncommon in geographic areas where a high endemic level Why ?

Dual infection with HBV and HCV is not uncommon in geographic areas where a high endemic level of both infections is reported, such as Southeast-Asia and Mediterranean. In general, the prevalence...

21 April 2018 1,041 0 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close