Does anyone have any recommendations about the First Choice RLM Race (Ambion) kit?

Gustavo Núñez-Acuña @Gustavo_Nunez-Acuna2

07 July 2012 1 4K Report

We have performed successfully 3-UTR amplifications, but we have issues with 5'-UTR.

cDNAs are from scallops. I have tested varying temperatures, dilutions of cDNA, but we don't have a good amplification. Do you have any tips?

Rashmi Gangwar

i am facing the same problem .. can u help me in troubleshooting

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Science method

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