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I'm running RNA nanochip R6K in the TapeStation 2200 instrument. The samples correspond to molluscs and crustaceans Total RNA. The issue we have is an overlapping of 18S and 28S peaks, therefore...
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We have performed successfully 3-UTR amplifications, but we have issues with 5'-UTR. cDNAs are from scallops. I have tested varying temperatures, dilutions of cDNA, but we don't have a good...
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