I am trying to choose between 2 transfection reagents knowing that I will be working with HepG2 cell line and HUH-7
Vendors are typically very happy to give a customer trial size aliquots of reagents so that researchers may try to see what works best for them. I suggest contacting the vendor to ask for a sample. Even the same reagents will often have differing transfection efficiencies, since cell-lines become "domesticated" in labs and phenotypes may alter on plastic.
Good luck!
Look for data regarding transfection efficiency (see https://altogen.com/product/huh-7-transfection-reagent-liver-cancer-cells/ for an example). You need to be sure that the reagents you are using will work for your cells, and the only way to be sure of it is to find actual data for the given cell type. There are lots of factors that affect reagent compatibility with given cell types, and with lipofection the variable concentration of various compounds in the reagent will definitely affect how well they can transfect liver cells.
Ibrahim,
Promega has already answered this. In fact, both Fugene 6 and Fugene HD have around 30% transfection efficiency in HePG2 and HuH7. Check Tables 1 and Table2 in https://worldwide.promega.com/resources/pubhub/tpub-205-choosing-the-right-transfection-reagent-for-optimal-efficiency/.
I have added a snapshot from Table 1 where Promega claims that their best transfection reagent for HepG2 and HuH7 is Viafect .
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