In my experiments, time has became an important variable associate to these proteins levels
Hi,
I don't know their half life which could be cell type specific. You can determine their half-life by cycloheximide chase assay.
Dear Gustavo,
The DKK1 mRNA half-life was estimated at 2.4 hours. For more details, please see:
Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain
Nicholas Polakowski1*, Heather Gregory1, Jean-Michel Mesnard2 and Isabelle Lemasson1*
Retrovirology 2010, 7:61 doi:10.1186/1742-4690-7-61
http://www.retrovirology.com/content/7/1/61
Hoping this will be helpful,
Rafik
Dear Gustavo,
The DKK1 mRNA half-life was estimated at 2.4 hours. For more details, please see:
Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain
Nicholas Polakowski1*, Heather Gregory1, Jean-Michel Mesnard2 and Isabelle Lemasson1*
Retrovirology 2010, 7:61 doi:10.1186/1742-4690-7-61
http://www.retrovirology.com/content/7/1/61
Hoping this will be helpful,
Rafik
Dear Gustavo,
The DKK1 mRNA half-life was estimated at 2.4 hours. For more details, please see:
Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain
Nicholas Polakowski1*, Heather Gregory1, Jean-Michel Mesnard2 and Isabelle Lemasson1*
Retrovirology 2010, 7:61 doi:10.1186/1742-4690-7-61
http://www.retrovirology.com/content/7/1/61
Hoping this will be helpful,
Rafik
Dear Gustavo,
The DKK1 mRNA half-life was estimated at 2.4 hours. For more details, please see:
Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain
Nicholas Polakowski1*, Heather Gregory1, Jean-Michel Mesnard2 and Isabelle Lemasson1*
Retrovirology 2010, 7:61 doi:10.1186/1742-4690-7-61
http://www.retrovirology.com/content/7/1/61
Hoping this will be helpful,
Rafik
- Badges
- Science method
- Similar topics
- Medicine
- Immunology
- Immunology Techniques
- Immunoassay
- ELISA