Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

Is it safe to open educational institutes amid new cases of Covid-19 without completion of vaccination??

Vaccination is in its First phase in India. The front line is to be vaccinated yet. In this situation opening of institutes may be dangerous for the life of children.

01 February 2021 660 2 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

Does behavior (shy vs bold; rare vs abundant) and flock size (small vs large) of bird species play role in niche partitioning among sympatric species?

Sympatric birds may avoid competition via multiple strategies - by different activity period, different diet, different microhabitat etc. Has any one looked into behavior of sympatric birds as...

11 January 2021 9,600 1 View

CPLEX Optimization tutorials ?

Respected all, Can anyone help me regarding where I can get complete and proper tutorials on IBM CPLEX Optimization software? I found some tutorials on Youtube...but they are just basic. I need...

05 January 2021 6,751 6 View

What is the correct formula to calculate NDWI ?

Gao (1996) gave the expression to calculate NDWI using Near Infrared (NIR) and Short wave Infrared (SIR), while some research suggests the use of the Green (G) band and Near Infrared (NIR) bands....

28 December 2020 9,938 2 View

How the problem of water scarcity in hilly region can be solved ?

the area received high rainfall, only last 3 months of hydrological year faces acute water shortage even for drinking the solution for this may be rainwater harvesting now how value...

25 December 2020 2,408 4 View

Which are the best methods to graft the polymeric nano/micro particles with lipids?

Good references (with clearly written methods/protocols) are highly appreciated. Polymers could be any, e.g., PLA, PCL, PLGA, etc. Similarly, lipids could be DSPE, DMPE, DMPA, DPPE, DLPC, DOTAP,...

07 December 2020 5,085 4 View

[SAP2000 related] I wonder why there is no any effect in the analysis result when I change the different parameters while modeling a frame structure?

(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...

06 October 2020 9,496 5 View

What is the most sensitive Non-invasive prenatal testing for fetal sex determination?

Is 8th week the earliest week where we can determine the fetal sex ?

05 October 2020 9,576 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View