Does anyone have any Esophagous cell lines?

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Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the relationship between the threshold voltage and the device's noise performance, particularly in terms of flicker noise and thermal noise?

The threshold voltage (Vth) of a MOS device plays a crucial role for its operation. At the same time, noise is an intrinsic factor. So how noise (flicker or thermal) change with the change in...

29 July 2024 3,246 0 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Does my paper explain the process of entanglement without resorting to retro causality or infinite branching universes?

In my paper, "Emergent Universe from Many Unreal World Interpretation ," I propose a novel interpretation of quantum mechanics that addresses the process of entanglement without relying on retro...

29 June 2024 10,045 5 View

Single crystal X-ray diffraction data solution?

I am working in metallosupramolecular single and multi-cavity cages. I am seeking a person who can help me to solve the single-crystal X-ray diffraction data.

08 June 2024 4,128 1 View

Please provide code/software names for performing Interpretive Structure Modeling (ISM), MICMAC and ANP?

Hello connections, I need code files or software (free/academic) names that can perform MCDM techniques like Interpretive Structure Modeling (ISM), MICMAC, and ANP.

23 April 2024 2,138 0 View

What are the most effective strategies for integrating green spaces into Central Business Districts (CBDs) to mitigate Urban Heat Island Effect?

The effectiveness of these strategies in mitigating UHIs and the challenges of implementing them in dense urban environments could also be addressed, This discussion is relevant to urban planners,...

17 April 2024 8,893 0 View

How do you interpret median prevalence?

How would you interpret the following sentence - " Global median rate of community residential facilities is 0.008 per 100000 population, there is presently a median of zero facililities per...

26 March 2024 8,945 5 View

Any workshop and conference related to Community Mental Health in USA?

I am interested in Community Mental Health - the following are my interest in community mental health- 1. Substance Use Disorders 2. mhGAP 3. Mental Health in older people 4. Stigma 5. Common...

05 March 2024 2,998 0 View

How to calculate the EMI SE in DB?

Inquiry Regarding Raw Data of S-Parameters and Calculation of EMI SE I am writing to discuss some aspects of the raw data we've acquired concerning S-parameters, specifically S11 and S21, obtained...

07 February 2024 1,038 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View