11 January 2015 1 9K Report

Do anyone have an idea what is the basis of taking ratio of DNA/envelope/packaging for Lentivirus preparation? My target vector is of 14kb but I don't know what rail should I take for my 2nd generation construct of DNA:pxPas2:VSVG. Unlimited ratios are defined in literature but iI don't know how to choose the best ratio for 150mm dish and to 6 well plate.

Thanks

Syed

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