Hongtian Stanley Yang

4 Questions 10 Answers 0 Followers

Questions related from Hongtian Stanley Yang

What's your protocol for isolating brain myeloid cells (including microglia and macrophage) by FACS?

I tried to isolate myeloid cells (microglia and macrophage) from adult mouse brain. Then I sorted CD11b and CD45 double positive cells through flowcytometer. However, the viability of the sorted...

05 May 2017 9,968 8 View

What is the protocol for preparing western blotting sample without adding reducing agent?

I used the antibody (against 14-3-3 theta) for immunoprecipitation (IP). When I use the same antibody to blot the sample from IP, it seems the light chain overlaps with the target band of 14-3-3....

23 February 2016 2,230 2 View

What's your favorite company for epigenetic modification screening?

I'm going to send rat cortical cultures (control and treatment) to a company for epigenetic modificaiton screening, including both histone modification and DNA methylation. Has anyone used the...

15 January 2015 7,891 7 View

Does anyone know the bacterial resistance of pAcYM1 plasmid?

Ampicilin or Kanamycin etc? I would also appreciate it a lot if someone can provide me with the map or literature addressing that issue. Thank you!

03 December 2014 1,093 3 View