Syed Raza

8 Questions 24 Answers 0 Followers

Questions related from Syed Raza

What is the dimeter of the 72h chick embryo capillaries?

I am studying the migration of nanoparticles in a 72hours chick embryo model. However I am not sure about the diameter of the capillaries of 72 hours chick embryo. I want to start with...

22 April 2016 9,146 3 View

Should I use CRISPR- KO or shRNA KD to study the role of a specific protein during migration of Neural Stem Cells (NSCs)?

I am planning to examine the role of a specific protein during migration of Neural Stem Cells (NSCs). For the same I wish to either KD or KO that particular protein in NSCs. Can anyone...

15 May 2015 8,355 4 View

How should I select an expression plasmid for a lentivirus construct?

Hi, I am quite new to the plasmid selection guide. I wish to select a GFP plasmid for lentiviral assembly to finally transfect mouse cell, and also for an another study: shSOD1 expression plasmid...

26 March 2015 6,825 5 View

How can I get a knockout efficiency of 100% of my gene of Interest using Lentiviral-CRISPR?

Recently I used 2 CRISPR containing lentiviral for downregulating the same gene at two different exons in NSCs/NPCs. The western blot (densitometric ananlysis) showed 96% down-regulation in...

08 March 2015 2,699 6 View

How can I trace transplanted Stem Cells in vivo, which are not labelled for any color?

Hi All, A friend of mine from India has raised a concern since they don't have the transduction facility, what are the other ways to trace the transplanted Stem Cells in vivo in rodents if they...

28 February 2015 8,196 15 View

How do I solve my difficulties with neural progenitor stem cells lentiviral transduction?

Hi All, I am trying to transduce my NPCs with the mCherry lentivirus, but I am getting very few transduce cells (Red). My NPCs are already GFP transducer, herewith I am attaching few pictures...

02 February 2015 2,085 18 View

What is the problem with nanodrop consistent reading?

I am purifying psPAX2 & VSVG plasmid with invitrogen Megaplasmid kit but the problem is when I am taking the reading for the sample, there is a huge difference. I am taking the reading 4 times...

19 January 2015 566 11 View

Does anyone have an idea what is the basis of taking ratio of DNA/envelope/packaging for Lentivirus preparation?

Do anyone have an idea what is the basis of taking ratio of DNA/envelope/packaging for Lentivirus preparation? My target vector is of 14kb but I don't know what rail should I take for my 2nd...

11 January 2015 8,904 1 View