14 Questions 44 Answers 0 Followers
Questions related from Syed Raza
Are there any specific markers for recognizing the stages of Alzheimer (mild, severe etc) in rodents. I wish to induce the same using streptozotocin, and transplant stem cells both at an early and...
25 April 2016 5,999 3 View
I am studying the migration of nanoparticles in a 72hours chick embryo model. However I am not sure about the diameter of the capillaries of 72 hours chick embryo. I want to start with...
22 April 2016 9,158 3 View
I am starting up a new experiment on autophagy, and wish to use an inhibitor for the same. Numerous articles are their both for Rapamycin as well as Choloroquine? Could anyone please be so kind to...
29 March 2016 3,649 3 View
I am planning to examine the role of a specific protein during migration of Neural Stem Cells (NSCs). For the same I wish to either KD or KO that particular protein in NSCs. Can anyone...
15 May 2015 8,359 4 View
Hi, I am quite new to the plasmid selection guide. I wish to select a GFP plasmid for lentiviral assembly to finally transfect mouse cell, and also for an another study: shSOD1 expression plasmid...
26 March 2015 6,834 5 View
I have to select my CRISPR KO cells which will show the best result from NPCs. For the same I wish to do single cell cloning for NPCs, but I am afraid that single cell cloning may lead to...
10 March 2015 4,954 5 View
Recently I used 2 CRISPR containing lentiviral for downregulating the same gene at two different exons in NSCs/NPCs. The western blot (densitometric ananlysis) showed 96% down-regulation in...
08 March 2015 2,707 6 View
Hi All, A friend of mine from India has raised a concern since they don't have the transduction facility, what are the other ways to trace the transplanted Stem Cells in vivo in rodents if they...
28 February 2015 8,205 15 View
Recently, I did a kill curve study for my NPCs. It was found that 0.5ug/ml was fine for the cells to die within 3-7 days? Now, I wish to ask can I challenged my NPCs (which are transduce with the...
17 February 2015 3,595 2 View
Hi All, I am trying to transduce my NPCs with the mCherry lentivirus, but I am getting very few transduce cells (Red). My NPCs are already GFP transducer, herewith I am attaching few pictures...
02 February 2015 2,099 18 View
Hi, I am working on Neural Progenitor Stem Cells, and presently making a CRISPR lentivirus for my NPCs studies. I have gone though a lot of protocols for the lentivirus preparation, and noticed...
01 February 2015 9,316 14 View
I am purifying psPAX2 & VSVG plasmid with invitrogen Megaplasmid kit but the problem is when I am taking the reading for the sample, there is a huge difference. I am taking the reading 4 times...
19 January 2015 571 11 View
Do anyone have an idea what is the basis of taking ratio of DNA/envelope/packaging for Lentivirus preparation? My target vector is of 14kb but I don't know what rail should I take for my 2nd...
11 January 2015 8,960 1 View
I am trying to make Lenivirus for my CRISPR construct, but I am using DMEM, without FBS but with P/S and not the OptiMEM medium. And Also I am using CaPo4 method instead of Lipofectamine 3000? But...
09 January 2015 3,399 23 View
