Hi All,
I would like to check whether commercially available cryovials with silicone gasket, internal thread are safe enough to store in liquid phase of nitrogen tank or not. I see all labs are practicing their own way. They said no problem and can dip into it. Some said no, the liquid can go into no matter with silicone seal or inner or outer thread and suggested to store in vapour stage instead. (above the liquid). Available cryoflex is not also convenient to use as it may take time to do it and still exist tiny spaces for liquid to come in too. Very confusing. What is the best method to seal the vial before putting into the cryobox of LNT. Are there any special seal? How about wax? Aluminium tape? or Silicone paste to wrap. Paper tape?