Hi everyone,
I am currently using an antibody from Merck (AB152, Lot # 2388829) .
I have tried different unmasking conditions (pH 6/9 manually and pH low/high with PT link), incubating temperatures (RT, 4°) and times (1h, 2h, ON), with our without permeabilization.
I don't detect any signals (I revealed my staining with AEC and DAB), so far I have tried this staining on cortex and adrenal glands.
If anyone have some tips, they are more than welcome.
Thank you for your help
Hi Laila,
I can only tell you what has worked for me. I use low pH Sodium citrate, boil sections for 10 min, then allow to cool for 20 min.
As for brain though I have little experience. Here is one of the references on the product page that may be helpful...
http://www.ncbi.nlm.nih.gov/pubmed/25794171
NM
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