I am conducting a study using Artemia salina as a model organism for algal toxicity. Previously I had only raised artemia to the point of hatching (24-48 hrs) and used them for feeding larval fish. I now want to raise them to older ages, meta-nauplii stage (in which digestive track has formed) and adult. To keep consistent with the conditions preferred by the algae I am studying, the artemia will be hatched and raised in 25 ppt artificial seawater in an incubator set to 25* C 12:12 light, dark cycle. Any advice on distinguishing between the many molts phases this species progresses through before reaching adulthood would also be helpful.
I have raised them to adult stage using baker's yeast and a mixed culture of microalgae. They can be maintained at adult stage with daphnia and microalgae.
1) For better results & growth, culture them directly under sunlight. Maintain a constant salinity of 35 ppt.
2)You can give rice bran extract,buttermilk (without any fat),extract of Caulreppa, Enteromorpha,whey powder suspended in water as feed,twice a day,ascertaining that turbidity is kept at a low level.
3) Adult can be raised in a span of about 15 days.Care to be taken to see that they are not disturbed-as no airation is required at any point of time.Do not raise the salinity of water +/- 5 ppt in a day,as they are not accustomed to such changes.
4) You can raise them with microalgae- but with out having any chaeta, as they block the fag end of their alimentary system- leading to their death.
But do you mean Artemia salina? American brine shrimp, A. franciscana, is the species used in most studies. A. salina is a species restricted to the Mediterranean region and Africa, and is actually threatened by the spread of A. franciscana through aquaculture. See e.g. the following papers
https://peerj.com/articles/200/
http://link.springer.com/article/10.1007/s10750-013-1748-6
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