Does anyone have a protocol to detect proline content in plant tissues?

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Marta Ruiz Valdés

Please find attached the protocol that I used. Hope it helps.

Darshani Wijayawardhana

Dear Marta Ruiz,

Thank you very much for your kind support.

Regards!

Darshani

Dear Darshani,

You are welcome.

Could you please explain the way of doing aspiration as in protocol?

Thanks!

We did a centrifugation at 12000x for 5' and then we carefully took coloured phase using a pipet.

Thank you very much.

P. Pardha-Saradhi

Suggested Bates et al. protocol is still the best protocol for determing proline levels. Our research team is first to demonstrate accumulation of proline in plants exposed to heavy metal stress (Alia & Pardha Saradhi, 1991 - you may download the same from my ReserachGate account). You may download several other papers we published on proline.

I do not recommend centrifugation. You can withdraw toluene layer containing colured proline-ninhydrine complex carefully with a dry pipette. If you wish you may visit my lab at University of Delhi, as my scholars use this method frequently and you can directly have a practical experience.

dear P.Pardha-Saradhi,

Yuan-Yeu Yau

@ Pardha,

I think it should be much easier for your lab to make a short film (it can be even accomplished by using a iPhone) and put it on YouTube, so other scholars from different parts of the world can just learn it from the film without traveling the long way to visit a lab. Nowadays, a lot of Universities, companies, scholars are taking advantage of YouTube for teaching and other purposes. Just a thought. Best.

Dear Dr. Yau,

Thanks for your suggestion and we shall try.

You might like to see simplest and eco-friendly protocol for proline quantification that appeared online in Protoplasma (you may download the same from my ResearchGate site).