Thank you so much for answering. Actually this is a part of my research where in a way I could confirm the applicability of my synthesized compounds as biological imaging agents. Here I am planning to use the stained mammalian cells (probably blood cells) by incubating them in maximum tolerable concentrations of the compounds and observe under epifluorescence microscope. I need to image fixed cells. A fixing protocol might be useful if there is any. The compounds are believed to associate with nuclei.

If I am not very clear please be kind enough to mention.

Thank you

Thank you very much for your detailed explanation. It was really helpful.

Thanks.