Does anyone have a protocol / paper on CRISPR/Cas9 Mice generation (point mutation)?

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Fazlur Rahman

Hi Shaikh, if you want to make a specific point mutation, you are going to need to proceed with homology directed repair..for that you will need to inject a donor vector along with your cas9..

i like this protocol: http://www.nature.com/nprot/journal/v8/n11/full/nprot.2013.143.html

just follow the homology directed repair methods..

Kai Schönig

check out the homepage from addgene: https://www.addgene.org/CRISPR/guide/

very helpful!

Joanne Kamens

I was going to say check out Addgene's CRISPR guide which has lots of protocols but Kai beat me to it--thanks for spreading the word! Our nonprofit only makes it when scientists support Addgene. Note, and you can call an Addgenie to get advice on choosing a vector if that would be helpful.

browsing the Google group on the CRISPR System can be also quite helpful:

https://groups.google.com/forum/#!forum/crispr