M Haroon Shaikh

7 Questions 6 Answers 0 Followers

Questions related from M Haroon Shaikh

Is it possible to cross two different cell specific Cre mice that are floxed for the same gene?

I am hoping to get suggestions, input and feasibility concerning breeding of Cre/lox mice. To get the required phenotype I am plannig to cross a hematopoietic specific Cre (Vav1-Cre) with an...

13 October 2019 8,565 6 View

MHC-II KO bone marrow chimeras?

I have made the bone marrow chimeras from the B6.MHC-II KO bone marrow into B6.WT mice (6.5x106 T-cell depleted BM cells). The chimerism was sucessfull as the irradiated control died 9-12...

16 October 2017 4,743 1 View

Protocol for the isolation/enrichment of T-cells from mouse Lamina propria?

I am looking for a robust protocol for the isolation of T-cells from mouse lamina propria for FACS analysis.

31 August 2017 6,027 1 View

Optimal cytokine mix to induce MHC-II expression on non-hematopoietic cells?

I am planing in vitro culture of mice non-hematopoietic cells with T-cells to access T-cell activation and proliferation via CFSE dilution assay. I am seaching for the cytokine(s) that can induce...

31 March 2017 4,417 1 View

Why there is a loss of flourescent protein expression (mEOS2) 10 post viral transduction?

I am using pLVX (Clonetech) lentiviral vector with insert of mEOS2-PH-Akt (Pleckstrin homology domain of Akt), particularly to visualize T cell synapse in Jurkat's upon APC stimulation. 72 days...

09 November 2016 3,727 5 View

Does anyone have a protocol / paper on CRISPR/Cas9 Mice generation (point mutation)?

I am looking for an article or a robust protocol to induce a point mutation using CRISPR/Cas9-based genome editing in mice.

10 December 2015 2,726 4 View

Can anyone recommend a protocol for Jurkat T-cells stimulation with soluble anti-CD3 and anti-CD28 antibodies?

I am looking for a protocol for the stimulation of Jurkat T cells with soluble anti-CD3 and ati-CD28 antibodies. I am working on the T-cells stimulation and effects of on kinectics. In my protocol...

07 July 2015 2,158 2 View