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Questions related from M Haroon Shaikh
I am hoping to get suggestions, input and feasibility concerning breeding of Cre/lox mice. To get the required phenotype I am plannig to cross a hematopoietic specific Cre (Vav1-Cre) with an...
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I am looking for a robust protocol for the isolation of T-cells from mouse lamina propria for FACS analysis.
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I am planing in vitro culture of mice non-hematopoietic cells with T-cells to access T-cell activation and proliferation via CFSE dilution assay. I am seaching for the cytokine(s) that can induce...
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I am looking for an article or a robust protocol to induce a point mutation using CRISPR/Cas9-based genome editing in mice.
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I am looking for a protocol for the stimulation of Jurkat T cells with soluble anti-CD3 and ati-CD28 antibodies. I am working on the T-cells stimulation and effects of on kinectics. In my protocol...
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