Fazlur Rahman

3 Questions 22 Answers 0 Followers

Questions related from Fazlur Rahman

Does anybody have experience with using NEB stable supercompetent cells?

since my insert is unstable, i am using NEB stable supercompetent cells. After transformation I get clones but with no plasmid in it..im using recommended ampicillin (100ug/ml) to make my...

15 January 2016 8,545 12 View

How many bands am I supposed to get in a ligation reaction and why?

Hi, I am having problem with ligation. I am usually successful most cases in cloning. but this one gene giving me hard time..i am not getting any colonies or very few colonies with no insert..i...

06 December 2015 6,669 25 View

How can I solve this ligation problem?

hi, im getting no colonies or colonies with no insert. I think it is probably ligation issues. the insert are self ligating but no vector insert product..how can I fix it..no problem with...

04 December 2015 6,041 5 View