Which Molecular Imager GelDoc do you use? Do you have a program or reference strains of known ribotypes?
There are multiple ways of ribotyping; conventional, capillary electrophoresis. Each require different setup and are well described in literature. As with any typing method, if you want to do this in house, it is best to have a reference collection (e.g. Leeds-Leiden collection) that you can use for comparison. Alternatively, you can try to use a webbased system such as Webribo.
If I can extend the answer Dr Smits,
you can follow protocol described in: https://www.ncbi.nlm.nih.gov/pubmed/25679978.
PCR ribotyping based on capillary electrophoresis has a great resolution capacity, you can use the WEBRIBO database (is freely available) as suggested Dr. Smits or/and you can share the profiles with other reference labs (e.g. Leiden).
The sequencer you need you can use in genetic department in your hospital.
The efforts worth it!
Good luck,
Marcela
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