I'm intending to load liposomes with doxorubicin but, i don't want to use the transmembrane pH gradient method, could there be other better method of doing the loading?
You can use co-solvent method that will give you better loading. You first dissolve all your lipids, cholesterol and doxorubicin (dox) in an organic solvent such as choloroform. Then evaporate the solvent to form a thin film using a round bottom flask using a rotary evaporator. The final step is to add a buffer/aqueous solution and vortex the samples followed by extrusion using membrane filters of varying pore size. This method will get you nanoparticles with unilamellar vesicles loaded with dox.
Arun Iyer , thank you for the recommended protocol. A quick one, does extrusion affect the encapsulation efficiency of the loaded doxorubicin via the co-solvent method?