I want to determine whether some novel compounds can interfere with estradiol activity by using this Alkaline Phosphatase activity assay in Ishikawa cells.
Hello, everybody. I am looking to add a DNAse treatment step to my bacterial lysis (gram-negative) procedure for crude enzyme extraction because my lysate always ends up being too viscous. We...
14 April 2024 280 2 View
Are there any conditions where it would be possible to lyse gram-negative bacteria using the Promega PLB? I would need to lyse the cells to test for crude enzyme activity but I don’t have a...
30 January 2024 7,625 1 View
Hello, We have been running LDH assays on skin fibroblasts in conjunction with our MTT assays. Our hours of exposure range from 24h-96h. Media is aspirated and changed and cells are re-dosed...
06 October 2020 9,223 3 View
For hepatitis work
21 April 2019 7,116 0 View
To confrm the transfarability of management theory practices from one country to another country and from private to public sectors
31 May 2018 4,684 4 View
Research topics related to strategy and structure for management students (Master). Case study of Nigeria Universities
29 May 2018 3,089 2 View
I am running MTT cytotoxicity assays on marine mammal skin fibroblasts. Although my results have been good for many toxicants, PCB126 results have been poor. I run my assay in triplicate and in...
28 January 2018 8,229 1 View
My question is about the pros and cons of the decision of the ECCC regarding the Cambodia case (genocide) and what about the restoration measurements taken place for the victims after 30...
21 September 2017 435 0 View
I am having trouble getting my salts and glucose to dissolve in milliQ water. Basically, I am making Kreb's Henseleit buffer from scratch. I used a stir bar for 6 hours yesterday and mixed in one...
10 May 2017 3,952 2 View
i am writing a literature review about narcissistic personality disorder and it is very difficult to find valid treatments. Can you suggest me any? Thanks in advance!
31 March 2016 3,330 11 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View