Does anyone have a protocol for in solution double digestion of a protein sample using Trypsin and GluC?

05 May 2014 1 5K Report

I want to make a double digestion of beta-galactosidase using Trypsin and GluC. I need a protocol for this, and is it better to make the digestion using both enzymes at the same time or to start with one of them followed by the other?

Mohammed Hasanain

The activation of trypsonigen to become active trypsine

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