Bassel Sabbagh

5 Questions 16 Answers 0 Followers

Questions related from Bassel Sabbagh

What if heavy and light peptides do not co-elute together?

Hello, i ordered couple of isotopic labeled peptides (13C/15N) for absolute quantification of proteins. The first measurements show that the light one from protein standard and the heavy one do...

09 September 2016 1,152 5 View

Do you know a product for separating cystein containing peptides?

Hello together,I am interested in separating peptides based on their cystein residues and looking for commercial product for this purpose. It would be better if it can be done using magnetic beads...

04 April 2016 590 4 View

How can I determine which attributes to use by applying SVM-RFE?

I have some questions on classification using Support Vector Mahine Recursive Feature Extraction with WEKA. RFE gives a final ranking for the attributes however one needs a method to decide which...

02 February 2015 6,581 11 View

Can anyone suggest a method for hemoglobin removal from a culture media?

I want to remove hemoglobin from aspergillus cell culture supernatant. It has been used as a sole nitrogen source in order to make aspergillus produce proteases. I want to remove it in order to...

10 October 2014 4,991 10 View

Does anyone have a protocol for in solution double digestion of a protein sample using Trypsin and GluC?

I want to make a double digestion of beta-galactosidase using Trypsin and GluC. I need a protocol for this, and is it better to make the digestion using both enzymes at the same time or to start...

05 May 2014 5,371 1 View