Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Online miRNA target prediction tool and related- down stream pathways?

There are many miRNA target prediction softwares, can you help me from your experience which one is the best? What are the consideratios I must take? because I have no experience in it...

02 September 2020 6,714 5 View

Fault analysis with Simulink- abnormal response after fault clearance?

The attached simple model with SIMULINK after clearing the fault, the Simulink is having abnormal responses. Bus voltage V3 is oscillating. anyone facing that issue?

09 April 2020 2,882 3 View

Can I appeal against a journal editorial decision?

Can I appeal against a journal editorial decision?

28 November 2018 8,076 50 View

How to convert Ti (integral time) and Td (derivative time) to I and D parameter in function block parameter for PID controller (SIMULINK)?

I need help on my problem. I managed to tune my PID controller using Ziegler-Nichols continuous cycling method and able to determine my Kp, Ti (integral time) and Td (derivative time). But the...

22 May 2017 5,641 8 View

What is the difference between generalised additive model and polynomial regression?

13 June 2016 6,758 5 View

How can I put cluster labels in the Gaussian Mixture Model (GMM)?

I used GMM to cluster my data. It has 4 dimensions with 3 clusters. The GMM ran very well, and I got the mean and covariance matrix for each cluster. The problem is I don't know which label...

15 October 2014 7,213 5 View

Where can I find researchers interested in education reform and health problems affecting students caused by problems in education?

I'm extremely interested in issues concerning students' heath in educational settings such as stress levels in class rooms and how standardized testing affects drug use on campuses. I would like...

27 July 2013 7,421 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

Using LINK11 to Apply force in ANSYS APDL?

I'm trying to use LINK11 to apply a force as if it was a cable pulling a structure twowards a point in ANSYS APDL. When I solve the model, the deformation of the overall structure is far too...

30 January 2021 7,919 1 View

PME calculation on a GPU GROMACS?

Hi all I used the following script to run the simulation but for some reason, I was not able to assign GPU to do the PME calculation? How to do that? The size of the system is huge (600.000...

14 January 2021 7,441 1 View

[JAVA]How to fix exception in thread error when using array of objects?

I am doing atm gui for my project in java. In my opinion using array of objects is the best option to store customer data. When i try to access it in an ActionListener method, i get this error:...

06 January 2021 6,975 4 View

GROMACS: Cannot rename checkpoint file; maybe you are out of disk space?

Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...

03 January 2021 3,544 2 View

How can a nonlinear buckling square tube crash with the Riks method ?

Hello everyone Well, this is my first post here so I hope this news will allow me the pleasure of chatting with you on this topic. So, as a student engineer in mechanical engineering specializing...

14 December 2020 7,513 1 View

How can one resolve ''Failed to locate main Gaussian directory'''?

I have got a problem with launching the Gaussian program after the installation of Gaussianview 5.0.9 software. When launching the application, it gives a warning message, ''failed to locate main...

26 November 2020 8,507 4 View

Can authors be different than those listed during registration of a trial?

I have a question if anyone can answer it... For a randomized control trial, when I come to publish the results to a journal...can we add more authors than those originally registered as co...

25 November 2020 8,285 2 View

Does PROCESS v3.5 by Andrew F. Hayes with SPSS Statistics 27 on MacOS 10.15.7 work?

After installing the PROCESS v3.5 tool, I ran a mediation model 4 with three variables (N = 125). The warnings below showed up on the output window, and the status at the bottom shows, "Running...

15 November 2020 3,930 14 View