Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

How to design manholes of a stormwater tunnel?

I am exploring for the design code or manual and standard drawings of access and maintenance manholes of the storm water conveying tunnels. (i.e free flow tunnels not pressurized ones) but all of...

22 December 2020 2,590 4 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

SAS code for a "Split-plot in time" (based on CRD design)?

Dear all, I want to analyze a factorial split-plot in time using SAS. Factorial Experiment using Completely Randomized Design (CRD); Factor A: treatments (a1-a4) Factor B: harvest time,...

28 October 2020 3,515 4 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Journal vs conference publications, which one would you prefer ?

For sure journal publications have more weight than conference publications but i am asking about your preference. For example i like conferences a lot because i like to meet people and explore...

06 September 2020 7,468 42 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

Any reference to learn numerical methods for Viscoelastic compressible fluids(oil) (CFD)?

I am developing a solver to simulate compression of oil in shock absorber filled with viscoelastic compressible oil. The model will consider the loss due to viscosity which will decide the...

15 January 2021 1,707 1 View

How to implement truss element with no effect of compression in ansys workbench static structural?

I was trying to add ligaments in cervical spine geometry. There it is mentioned in the existing literature to add the ligaments in the geometry as truss element with no effect of compression....

03 January 2021 2,205 0 View

Why does bending stress(Sx) in ANSYS APDL direct below of point load FE 2D modelling simply supported point load higher than exact solution in value?

Why does normal bending stress/flexural stress value (Sx) just direct below a concentrated load of simply supported beam which is compression stress higher than the hand calculation Exact solution...

03 January 2021 8,462 3 View

Is the concrete tensile stress-strain curve a tenth of its compressive one ?

The known compressive stress-strain curve of concrete corresponds to the negative strains despite the selected axis shift to the positive strains values. The concrete tensile yield stress is known...

27 December 2020 7,309 7 View

What happens to a gas after compressing it for 10 years?

Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...

07 December 2020 6,150 3 View

How to apply the Orthogonal Matching Pursuit(OMP)Algorithm on images?

Hello to all, I am research scholar and pursuing Ph.D in the field of Compressive Sensing. I want to know , how to apply the OMP algo. for reconstructing the compressed image. Since i have done...

06 December 2020 8,012 6 View

Assessing quality of CPR?

In simulation practice/training, are there set standards for monitoring quality of a resuscitation attempt? beyond rate/depth of compressions and time off chest?

23 November 2020 5,182 12 View