Chromosome identification depends on their morphological characteristics such as relative length, arm ratio, presence and absence of secondary constrictions on the chromosome arms. Therefore, it is an additional and useful tool for the identification of individual chromosome within the chromosome complement. G bands correspond exactly to chromomeres of meiotic chromosomes but the mechanism leading to the visualization of the basic chromosome pattern is still unclear. The process is believed to be associated with denaturation and distributin of non-histone proteins and rearrangements of chromatin fibres from G negative to G positive bands. Try to have a pictures of each slide and cut every chromosome and compare it to the others as in Karyotype.

First, find the characteristics of the karyotype of the organism that you are studying and on it compare and identify your preparations. The easiest way to do this is with karyotyping software.

I assume that you are in the area of General Genetics and that you have done introductory courses that have exposed you to chromosome forms. You will read more about karyotyping and also do good chromosome spreads and master the art of recognising their forms. There are manuals that teach this and there are Descriptors that prescribe the standards.

My honest advise is that you should learn hands-on and master the basic skills. Softwares are good but you will never be able to vet the products of your software if you don't empower yourself.

Good one @Julius. However, AI people will not agree with you who work hard to automate the analysis at least. One needs to understand the basic principles as highlighted above.