How to calculate the internal pressure of a riser pipe?

I am designing a riser pipe for use in ocean down to depths of 5km. Polymetallic nodules are pumped from the seabed to the surface. For my FEA and CFD analyses I need to input a maximum pressure...

06 February 2021 2,803 3 View

Looking for an Electronics Engineer working in the field of Materials Science?

Hello, I have an assignment in my material science class that requires us to interview an electronics engineer that practices material science at work. I heard manufacturing engineers at...

16 November 2020 9,318 3 View

Magnetite ultrafine powder supplier?

Looking for a supplier that has 25 micron max particle size as an available option. Found one, but they won't even sell us a sample because they don't think it will work for us. Crazy! All I...

24 August 2020 2,752 5 View

What kind of research topic for industrial engineers take at the time of COVID 19 ?

As we know in this pandemic, everything seems quite hard to control. I hope the researches could discuss and share their thoughts for researching at this time. Especially related in Industrial...

27 July 2020 2,360 7 View

GFP antibody for flow cytometry?

Hello, I have been trying to detect the YFP expression in my YFP-tagged mouse model with anti-GFP antibody for flow cytometry. My question is, is this normal to observe a shift in GFP expression,...

23 July 2020 4,300 12 View

Any recommendations of journals to publish bacterial genome announcements?

I am looking for a journal to publish microbiological/bacterial genome announcements for now and in the future.

17 May 2020 3,010 8 View

Is it okay to use latent variables in the SEM model that have different constraints than CFA for individual latent variables?

I am conducting a structural equation model with three latent variables containing three indicators each. The latent variable CFA's are significant with constraints on 2/3 indicators, but the SEM...

28 April 2020 3,275 4 View

Is secondary fixation necessary for processing plant materials for SEM?

I am trying to create a protocol for several fixation methods for Scanning electron microscopy. using FAA, glutaraddehyde and ethanol as fixatives.

11 November 2019 7,215 1 View

Why won't my freshly isolated primary turkey hepatocytes attach?

We use a collagenase perfusion method to isolate hepatocytes from turkeys. We are culturing them in monolayer with the ultimate goal of using them to evaluate drug metabolism. The experiments were...

03 October 2019 5,662 1 View

What are the stylistic conventions for literature reviews in interdisciplinary papers that use grounded theory?

If you are using grounded theory to extract patterns from your data and you find data that correlates with pre existing theories, is it acceptable to add them to your results and section, or...

09 February 2018 9,160 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View