I am working in gene expression studies of walking catfish (Clarias batrachus). We use in vivo models for the treated conditions. But to study the inhibitor effects I need to have primary cell culture. So I would be grateful if anyone can provide primary hepatocytes or primary kidney cell line!
Stéphane Roche
If it is like human cells, you must prepare it. Often differenciated cells can not be maintain in culture, and particulary hepatocytes and epithelial cells.
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Maryam Hazeri
I tried several time for preparing primary cell line from some fish, it is a bit difficult but you should attention about the degree around trypsinized tissue
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