Maryam Hazeri

4 Questions 11 Answers 0 Followers

Questions related from Maryam Hazeri

Can conventional PCR primers be used for qPCR?

Can I use conventional PCR primers for qPCR for virus quantification? i don t have any experience for qPCR and I got good band by using those primers in conventional PCR , MCP gene was cloned into...

05 May 2015 4,034 3 View

How can I design standard curve with cDNA for qPCR?

I will be performing qPCR with cDNA templates. Is it okay to use a genomic DNA for desing my standard curve by cDNA dilutions? What are the drawbacks and/or benefits of it?

03 March 2015 637 10 View

Does anyone have experience with primary culture in fish?

I did primary culture with caudal fin of fresh water fish according primary fish culture protocol with L-15 media and 15% FBS but the cells did not attach,could you please help me if you have any...

02 February 2014 7,855 7 View

How to culture cryopreservative SSN-1 cells?

I cultured cryopreservative SSN-1 cells in L-15 +15% FBS +penstrep+ kanamycine, but after checking after 5 days they did not attach- is there any reason for that?

11 November 2013 8,632 19 View