Bodhisattwa Banerjee

6 Questions 29 Answers 0 Followers

Questions related from Bodhisattwa Banerjee

Is it necessary to run a SYBR green based real-time PCR product in agarose gel?

To check the primer specificity we do run a melt curve analysis and a single curve implies the formation of a single product. But I have seen in many papers people reconfirming the specificity by...

01 January 2017 3,329 10 View

Does anyone have fish carbamoyl-phosphate synthetase III (CPS III) antibody?

I am working on the expression of ornithine-urea cycle genes and proteins in fish. But the problem is that we do not have fish-specific CPS III available commercially. So if anyone has CPS III...

04 April 2014 1,552 1 View

How can we reduce the frothing at the time of homogenising the tissue?

Whenever we homogenise the tissues for preparing samples for Western blot or any other experiment (i.e. enzyme assay, etc.), a lot of frothing occurs. How can we decrease the amount of frothing?...

03 March 2014 7,462 4 View

Does anyone have a primary cell line of walking catfish (Clarias batrachus)?

I am working in gene expression studies of walking catfish (Clarias batrachus). We use in vivo models for the treated conditions. But to study the inhibitor effects I need to have primary cell...

01 January 2014 8,696 2 View

Can anyone please explain why my qPCR results are showing exponential algorithm failed for a particular gene?

Whenever I am trying to run a qPCR analysis for a particular gene the exponential algorithm is failing. I have tried two different set of primers. Although the amplification curve is coming nicely...

09 September 2013 7,918 7 View

How do you titrate the antibody for immunocytochemistry?

Can anyone please explain how should I titrate the optimal antibody dilution for immunofluorescence using CY3 conjugated secondary antibodies?

09 September 2013 1,296 24 View