How to regenerate PES Membrane of centrifugal filtration tubes having 5 and 50kDa MWCO?

Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...

15 December 2020 7,951 3 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

How I can simulate solid state sintering?

I want to simulate solid state sintering of metal powder, conducted under vacuum. I am interested in the initial stage of sintering, does not matter to reach full density. Do you know any model or...

24 September 2020 7,383 5 View

Is there a good library to perform Petri net simulation in python?

Hello, I have designed several petri nets for my research. I am wondering how can I simulate them and gather system performance measure (eg sojourn times, throughput) using python? Is there a...

11 May 2020 2,120 2 View

What are the relevant economic, technical and environmental aspects of the recycling of batteries for automotive applicantions?

A paper review. Could you please provide some researches about the topic?

28 April 2020 946 5 View

Difference between photovoltaic plant short-circuit current and synchronus generator short-circuit current in AC transmission system?

Hello, I understand that photovoltaic plants are able to disconnect very quickly if there is a fault in the grid (cause power electronics), while synchronus generators cant disconnect due to his...

30 October 2019 8,457 5 View

Psychic salience has been studied in his correlation with psychosis.What about psychotropic drugs effect?

Some drugs seems to raise the feeling of psychic salience while others dont.

20 September 2018 3,556 1 View

In psychotic patients, psychotic features of the speech can vary their gravity during an interview. What do you think can be the influential factors?

In my view, these factors can be: the emotional meaning of the theme for the patient, anxious sympyoms caused by the situation or the interviewer, a relatedness feeling with the interviewer, a...

03 September 2018 303 3 View

Which assay to detect human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies?

We are currently using indirect ELISA to measure human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies? This indirect ELISA is not sensitive enough for our...

23 May 2018 6,546 2 View

Ksp in aprotic polar solvent , any idea?

11 May 2018 2,772 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View