Does anyone have a good protocol for running Native PAGE?

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Ewa Brzozowska

by davis

F Luis González Flecha

Blue native PAGE, Nat Protoc. 2006;1(1):418-28. It works fine, even with hydrophobic membrane proteins.

http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.62.html

By the way, size exclusion chromatography (e.g. Superdex 75 or 200) coupled with a Light scattering detector is a better option to check the monomeric state.

Tesmine Martin

Thank you

Thank you Bernd!