My protein has a PI in the physiological pH and is in the phosphate buffer, pH 6.0. I would like to check if the protein is in the monomeric state.
F Luis González Flecha
Blue native PAGE, Nat Protoc. 2006;1(1):418-28. It works fine, even with hydrophobic membrane proteins.
http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.62.html
By the way, size exclusion chromatography (e.g. Superdex 75 or 200) coupled with a Light scattering detector is a better option to check the monomeric state.
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