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My peptide is 3.6kDa. After labeling with Alexafluor 647 C2 maleimide dye, is there an efficient way to remove the unreacted dye?
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At pH 6.0 DTT does not exist in its thiolate form. As thiolate form of DTT initiates the reduction process, can it still perform its function at pH 6.0? Or are there any other alternative reducing...
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My protein has a PI in the physiological pH and is in the phosphate buffer, pH 6.0. I would like to check if the protein is in the monomeric state.
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