Nasir Abbas

4 Questions 10 Answers 0 Followers

Questions related from Nasir Abbas

What is activation marker of Kupffer cells (in specific) or activation marker of macrophage (in general)?

I am working on localized liver injury model in mouse to study recruitment of inflammatory immuno cell types and then specifically detecting these cell types with IHC-p. I was using F4/80 marker...

19 June 2017 4,438 1 View

Effect of over fixation in 4% PFA on antigen integrity?

The antigen of interest I am going to detect (Paraffin embedded) is expected to be low in abundance. On the top of that my experiment demands use of mouse liver tissue fixed in 4% PFA for more...

23 November 2016 8,523 7 View

RNA Isolation from backup tissue?

whether liver back up tissue stored in liquid nitrogen WITHOUT Trizol reagent (normally used for protein extraction) could be used for RNA extraction? Any one ever had encountered such case?

09 August 2016 4,990 3 View

Any advice on endogenous Biotin quenching?

Instead of using commercial Avidin Biotin Complex, is there any conventional method/recipe to quench endogenous biotin in liver sections? Would be delighted to know!

30 December 2015 2,317 3 View