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Questions related from Nasir Abbas
Going to deplete macrophages in mice liver by clodronate liposomes (0.1mL/10gm) to check an effect; 1) 48hrs and 72hrs after an injury and then 2) Repopulating the macrophages by adoptive transfer...
07 July 2017 9,904 0 View
I am working on localized liver injury model in mouse to study recruitment of inflammatory immuno cell types and then specifically detecting these cell types with IHC-p. I was using F4/80 marker...
19 June 2017 4,525 1 View
The antigen of interest I am going to detect (Paraffin embedded) is expected to be low in abundance. On the top of that my experiment demands use of mouse liver tissue fixed in 4% PFA for more...
23 November 2016 8,588 7 View
whether liver back up tissue stored in liquid nitrogen WITHOUT Trizol reagent (normally used for protein extraction) could be used for RNA extraction? Any one ever had encountered such case?
09 August 2016 5,042 3 View
In double immunoflourescence experiment by confocal, I intend to use two conjugated anti-cd11b and anti-f4/80 Ab (PE and FITC) in mice liver sections. I need your opinion whether, 1) Conjugated Ab...
11 July 2016 3,951 3 View
Instead of using commercial Avidin Biotin Complex, is there any conventional method/recipe to quench endogenous biotin in liver sections? Would be delighted to know!
30 December 2015 2,369 3 View
