I'm trying to do dot blots using m6A antibody from synaptic systems (https://www.sysy.com/products/m6a/facts-202003.php). So far I tried nitrocellulose membranes and Hybond N+ membranes. Also I tried blocking with ELK and BSA. However so far, the background stays relatively high compared to my signal.
Are there other things I can try to improve my blots?