Lucas Picavet

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Questions related from Lucas Picavet

Does anyone have a good protocol for doing m6A dot blots with RNA samples?

I'm trying to do dot blots using m6A antibody from synaptic systems (https://www.sysy.com/products/m6a/facts-202003.php). So far I tried nitrocellulose membranes and Hybond N+ membranes. Also I...

18 September 2017 7,804 2 View

How comparable are THP1 cell lines to primary monocytes isolated from PBMCs?

For our experiments we use THP1 cell lines to manipulate genes and see what happens to their expression of cytokines with and without LPS stimulation. We use this approach since primary monocytes...

28 April 2017 4,938 1 View