9 Questions 7 Answers 0 Followers
Questions related from Anil Kiran Chokkalla
I am performing ChIP-Seq experiment for REST, H3K9Ac and H3K9Me2 marks in rat cortical tissues. I successfully made the ChIP libraries. At this point, I am wondering about the sequencing depth for...
08 September 2017 3,513 3 View
I was trying to analyse expression levels of certain genes after treatment of monocytic THP1 cell line with antigenic proteins through qPCR and i tried using the reference genes beta actin and...
11 August 2014 2,148 6 View
I am trying to isolate RNA from THP1 cell line using manually made TRIZOL reagent, I am getting good intact RNA but along with it I am getting huge DNA contamination. So I want to somehow minimise...
11 August 2014 8,026 9 View
There are many methods for quantification of gene expression (fold change calculations) through real time PCR such as Pfaffl method, delta ct method, standard curve method. So I want to know which...
11 August 2014 3,083 4 View
I want to know the reason why interactions between translation termination factors and EJCs lead to mRNA decay during NMD.
01 November 2013 8,319 0 View
What is the role of Poly(A) in conferring mRNA stability?
31 October 2013 1,740 2 View
The role of RNA helicase activity of Upf1 in NMD.
29 October 2013 8,703 4 View
Difference between Internal Ribosome Entry Sites and kozak sequences.
20 October 2013 801 4 View
I am studyingcombination therapies in TNBC and I want to know weather MDA-MB-453 belongs to TNBC sub type.
28 September 2013 7,787 13 View