Anil Kiran Chokkalla

9 Questions 7 Answers 0 Followers

Questions related from Anil Kiran Chokkalla

Sequencing depth for ChIP-Seq analysis of REST (transcriptional repressor), H3K9Ac and H3K9Me2?

I am performing ChIP-Seq experiment for REST, H3K9Ac and H3K9Me2 marks in rat cortical tissues. I successfully made the ChIP libraries. At this point, I am wondering about the sequencing depth for...

08 September 2017 3,510 3 View

What is the best reference gene for normalising the expression level of a given gene of interest in qPCR experiment?

I was trying to analyse expression levels of certain genes after treatment of monocytic THP1 cell line with antigenic proteins through qPCR and i tried using the reference genes beta actin and...

11 August 2014 2,145 6 View

Does anyone have a composition of manually made TRIZOL reagent that worked well?

I am trying to isolate RNA from THP1 cell line using manually made TRIZOL reagent, I am getting good intact RNA but along with it I am getting huge DNA contamination. So I want to somehow minimise...

11 August 2014 8,023 9 View

Which is the most reliable method for quantification of gene expression data in qPCR experiment?

There are many methods for quantification of gene expression (fold change calculations) through real time PCR such as Pfaffl method, delta ct method, standard curve method. So I want to know which...

11 August 2014 3,079 4 View

How does the interaction of exon junction complex (EJC) and Upf proteins with translation termination factors lead to mRNA decay?

I want to know the reason why interactions between translation termination factors and EJCs lead to mRNA decay during NMD.

01 November 2013 8,316 0 View

How does Poly(A) at 3' end of mRNA confer stability to mRNA?

What is the role of Poly(A) in conferring mRNA stability?

31 October 2013 1,737 2 View

What is the purpose of ATP dependant RNA helicase activity of Upf1 in non-sense mediated mRNA decay(NMD)?

The role of RNA helicase activity of Upf1 in NMD.

29 October 2013 8,700 4 View

What is the difference between IRES and kozak sequences?

Difference between Internal Ribosome Entry Sites and kozak sequences.

20 October 2013 799 4 View

Is MDA-MB-453 cell line triple negative?

I am studyingcombination therapies in TNBC and I want to know weather MDA-MB-453 belongs to TNBC sub type.

28 September 2013 7,784 13 View