Dear Ilknur,

You can find your answer in some articles as follows:

http://idosi.org/wasj/wasj16%283%2912/1.pdf

http://scholarsresearchlibrary.com/ABR-vol4-iss1/ABR-2013-4-1-271-278.pdf

http://omicsonline.org/scientific-reports/2155-9910-SR-678.pdf

http://kasetsartjournal.ku.ac.th/kuj_files/2009/a0908211108575625.pdf

http://www.ijppsjournal.com/Vol2Issue4/744.pdf

http://link.springer.com/article/10.1007%2Fs12257-013-0316-2

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791228/

http://faculty.mu.edu.sa/public/uploads/1338113774.7306projectpdf%5B1%5D.pdf

The last reference is a thesis submitted for the degree of Postgraduate Diploma in Science At the University of Otago, Dunedin, New Zealand.

Hopefully it would be useful for you.

Dr. Zorriehzahra,

Thank you very much for your help. I m appreciate you.

Bests,

Determine as hydroxyproline content

i do have a method for hydroxyproline . Mail me

Collagen content can be determined by carrying out hydroxyproline assays.  Therefore to obtain the amount of collagen in a sample; in mammals the amount of hydroxyproline in the sample (mg) is multiplied by a factor of 7.46, whereas for aquatic animals, the hydroxyproline content is multiplied by a factor 14.70.